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- Title
Formulations of the endophytic bacterium Tu-100 suppress on oilseed rape and improve plant vigor in field trials conducted at separate locations.
- Authors
Hu, Xiaojia; Roberts, Daniel P.; Maul, Jude E.; Emche, Sarah E.; Liao, Xing; Guo, Xuelan; Liu, Yeying; McKenna, Laurie F.; Buyer, Jeffrey S.; Liu, Shengyi
- Abstract
causes serious yield losses in crops in the People's Republic of China. Two formulations of oilseed rape seed containing the bacterium Tu-100 were evaluated for suppression of this pathogen in field trials conducted at two independent locations. The pellet formulation significantly reduced disease (incidence and disease index) and increased plant dry mass, while the wrap formulation significantly reduced disease incidence and significantly increased plant dry mass at both field locations. Mean seed yield per 120 plants with both formulations of isolate Tu-100 was significantly greater than the appropriate controls, but at only one of the locations. Both formulations provided stable B. subtilis Tu-100 biomass (≥105 CFU·g-1) and seed germination (≥85%) over a 6 month period at room temperature. Polymerase chain reaction and DNA sequence analysis identified ituC and ituD, and bacAB and bacD in the genome of isolate Tu-100. These genes are involved in the biosynthesis of iturin and bacilysin. Iturin was detected in culture filtrates from isolate Tu-100, with thin layer chromatography. Detection of bacilysin was not attempted. Experiments reported here indicate the commercial viability of B. subtilis Tu-100 for suppression of S. sclerotiorum on oilseed rape.
- Subjects
OILSEED plants; CROP losses; FIELD research; PLANT-pathogen relationships; PLANT diseases; POLYMERASE chain reaction; NUCLEOTIDE sequence; BACILLUS subtilis
- Publication
Canadian Journal of Microbiology, 2011, Vol 57, Issue 7, p539
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/w11-041