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- Title
Direct activating effects of adreno cortico tropic hormone (ACTH) on brown adipose tissue are attenuated by corticosterone.
- Authors
van den Beukel, Johanna C.; Grefhorst, Aldo; Quarta, Carmelo; Steenbergen, Jacobie; Mastroberardino, Pier G.; Lombes, Marc; Delhanty, Patric J.; Mazza, Roberta; Pagotto, Uberto; van der Lely, Aart Jan; Themmen, Axel P. N.
- Abstract
Brown adipose tissue (BAT) and brown-like cells in white adipose tissue (WAT) can dissipate energy through thermogenesis, a process mediated by uncoupling protein 1 (UCP1). We investigated whether stress hormones ACTH and corticosterone contribute to BAT activation and browning of WAT. ACTH and corticosterone were studied in male mice exposed to 4 or 23°C for 24 h. Direct effects were studied in T37i mouse brown adipocytes and primary cultured murine BAT and inguinal WAT (iWAT) cells. In vivo effects were studied using 18F-deoxyglucose positron emission tomography. Cold exposure doubled serum ACTH concentrations (P=0.03) and fecal corticosterone excretion (P=0.008). In T37i cells, ACTH dose-dependently increased Ucp1 mRNA (EC50=1.8 nM) but also induced Ucp1 protein content 88% (P=0.02), glycerol release 32% (P=0.03) and uncoupled respiration 40% (P=0.003). In cultured BAT and iWAT, ACTH elevated Ucp1 mRNA by 3-fold (P=0.03) and 3.7-fold (P=0.01), respectively. In T37i cells, corticosterone prevented induction of Ucp1 mRNA and Ucp1 protein by both ACTH and norepinephrine in a glucocorticoid receptor (GR)-dependent fashion. ACTH and GR antagonist RU486 independently doubled BAT 18F-deoxyglucose uptake (P=0.0003 and P= 0.004, respectively) in vivo. Our results show that ACTH activates BAT and browning of WAT while corticosterone counteracts this.
- Subjects
ADRENOCORTICOTROPIC hormone; BROWN adipose tissue; WHITE adipose tissue; CORTICOSTERONE; POSITRON emission tomography
- Publication
FASEB Journal, 2014, Vol 28, Issue 11, p4857
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.14-254839