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- Title
Uric acid induces endothelial dysfunction by vascular insulin resistance associated with the impairment of nitric oxide synthesis.
- Authors
You-Jin Choi; Yujin Yoon; Kang-Yo Lee; Tran Thi Hien; Keon Wook Kang; Kyong-Cheol Kim; Jeewoo Lee; Moo-Yeol Lee; Seung Mi Lee; Duk-Hee Kang; Byung-Hoon Lee
- Abstract
Endothelial dysfunction is defined as impairment of the balance between endothelium-dependent vasodilation and constriction. Despite evidence ofuric acid-induced endothelial dysfunction, a relationship with insulin resistance has not been clearly established. In this study, we investigated the role of vascular insulin resistance in uric acid-induced endothelial dysfunction. Uric acid inhibited insulin-induced endothelial nitric oxide synthase (eNOS) phosphorylation and NO production more substantially than endothelin-1 expression in HUVECs, with IC50 of 51.0, 73.6, and 184.2, respectively. Suppression of eNOS phosphorylation and NO production by uric acid was PI3K/Akt-dependent, as verified by the transfection with p110. Treatment of rats with the uricase inhibitor allantoxanamide induced mild hyperuricemia and increased mean arterial pressure by 25%. While hyperuricemic rats did not show systemic insulin resistance, they showed impaired vasorelaxation induced by insulin by 56%. A compromised insulin response in terms of the Akt/eNOS pathway was observed in the aortic ring of hyperuricemic rats. Coadministration with allopurinol reduced serum uric acid levels and blood pressure and restored the effect of insulin on Akt-eNOS pathway and vasorelaxation. Taken together, uric acid induced endothelial dysfunction by contributing to vascular insulin resistance in terms of insulin-induced NO production, potentially leading to the development of hypertension.
- Subjects
ENDOTHELIUM; VASODILATION; INSULIN research; NITRIC oxide; PHOSPHORYLATION
- Publication
FASEB Journal, 2014, Vol 28, Issue 7, p3197
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.13-247148