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- Title
Insulin-stimulated Akt kinase activity is reduced in skeletal muscle from NIDDM subjects.
- Authors
Krook, Anna; Roth, Richard A.; Jiang, Xin Jian; Zierath, Juleen R.; Wallberg-Henriksson, Harriet; Krook, A; Roth, R A; Jiang, X J; Zierath, J R; Wallberg-Henriksson, H
- Abstract
The serine/threonine kinase Akt (PKB/Rac) has been implicated as playing a role in the insulin-signaling pathway to glucose transport. Little is known regarding the regulation of Akt kinase activity in insulin-sensitive tissues, such as skeletal muscle, or whether this regulation is altered in insulin-resistant states such as NIDDM. We examined the effect of insulin on Akt kinase activity in skeletal muscle from six NIDDM patients and six healthy subjects. Whole-body insulin sensitivity, assessed by the euglycemic-hyperinsulinemic clamp, was significantly lower in NIDDM subjects (P < 0.001), and this was accompanied by impaired in vitro insulin-stimulated glucose transport in skeletal muscle. In both groups, insulin induced a significant increase in Akt kinase activity, but the response to maximal insulin (60 nmol/l) was markedly reduced in skeletal muscle from NIDDM subjects (66% of control levels, P < 0.01). Impaired Akt kinase activity was not accompanied by decreased protein expression of Akt. Instead, a trend toward increased Akt expression was noted in skeletal muscle from NIDDM subjects (P < 0.1). These parallel defects in insulin-stimulated Akt kinase activity and glucose transport in diabetic skeletal muscle suggest that reduced Akt kinase activity may play a role in the development of insulin resistance in NIDDM.
- Subjects
INSULIN; PROTEIN kinases; MUSCLES; TYPE 2 diabetes; PHYSIOLOGY; CYTOCHEMISTRY; PROTEIN metabolism; GLUCOSE metabolism; BIOLOGICAL transport; COMPARATIVE studies; RESEARCH methodology; MEDICAL cooperation; REFERENCE values; RESEARCH; TRANSFERASES; EVALUATION research; SKELETAL muscle
- Publication
Diabetes, 1998, Vol 47, Issue 8, p1281
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diab.47.8.1281