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- Title
Up-regulation of plasma membrane-associated redox activities in neuronal cells lacking functional mitochondria.
- Authors
Dong-Hoon Hyun; Hunt, Nicole D.; Emerson, Scott S.; Hernandez, Joe O.; Mattson, Mark P.; de Cabo, Rafael
- Abstract
Mitochondria-deficient cells (ρo cells) survive through enhanced glycolytic metabolism in the presence of pyruvate and uridine. The plasma membrane redox system (PMRS) contains several NAD(P)H-related enzymes and plays a key role in maintaining the levels of NAD+/NADH and reduced coenzyme Q. In this study, ρo cells were used to investigate how the PMRS is regulated under conditions of mitochondrial dysfunction. ρo cells exhibited a lower oxygen consumption rate and higher levels of lactate than parental cells, and were more sensitive to glycolysis inhibitors (2-deoxyglucose and iodoacetamide) than control cells. However, they were more resistant to H2O2, consistent with increased catalase activity and decreased oxidative damage (protein carbonyls and nitrotyrosine). PM-associated redox enzyme activities were enhanced in ρo cells compared to those in control cells. Our data suggest that all PMRS enzymes and biomarkers tested are closely related to the ability of the PMs to maintain redox homeostasis. These results illustrate that an up-regulated PM redox activity can protect cells from oxidative stress as a result of an improved antioxidant capacity, and suggest a mechanism by which neurons adapt to conditions of impaired mitochondrial function.
- Subjects
MITOCHONDRIA; CELL membranes; PYRUVATES; URIDINE; NAD (Coenzyme); GLYCOLYSIS
- Publication
Journal of Neurochemistry, 2007, Vol 100, Issue 5, p1364
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2006.04411.x