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- Title
A role for caveolin-1 in post-injury reactive neuronal plasticity.
- Authors
Gaudreault, Sophie B.; Blain, Jean-François; Gratton, Jean-Philippe; Poirier, Judes
- Abstract
Remodeling and plasticity in the adult brain require cholesterol redistribution and synthesis for the formation of new membrane components. Caveolin-1 is a cholesterol-binding membrane protein involved in cellular cholesterol transport and homeostasis. Evidence presented here demonstrates an up-regulation of caveolin-1 in the hippocampus, which was temporally correlated with an increase in synaptophysin during the reinnervation phase in a mouse model of hippocampal deafferentation. Using anin vitromodel of neuronal reactive plasticity, we examined the effect of virally mediated overexpression of caveolin-1 on injured differentiated PC12 cells undergoing terminal remodeling. Three days post lesion, caveolin-1-overexpressing cells revealed increases in synaptophysin and GAP-43, two markers of neurite sprouting and synaptogenesis. Morphologically, caveolin-1-overexpressing cells showed a decrease in primary neurite outgrowth and branching as well as an increase in neurite density. Caveolin-1-overexpressing cells also revealed the presence of terminal swelling and beading along processes, consistent with a possible alteration of microtubules stability. Moreover, a focal enrichment of caveolin-1 immunofluorescence was observed at the bases of axonal and dendritic terminals of mouse primary hippocampal neurons. Altogether, these results indicate that caveolin-1 plays an active role in the regulation of injury-induced synaptic and terminal remodeling in the adult CNS.
- Subjects
NEUROPLASTICITY; NEUROPHYSIOLOGY; MICROTUBULES; IMMUNOFLUORESCENCE; LABORATORY mice; NEUROCHEMISTRY
- Publication
Journal of Neurochemistry, 2005, Vol 92, Issue 4, p831
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2004.02917.x