We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Antibiotic perturbation of mixed-strain Pseudomonas aeruginosa infection in patients with cystic fibrosis.
- Authors
Tai, Anna S.; Sherrard, Laura J.; Kidd, Timothy J.; Ramsay, Kay A.; Buckley, Cameron; Syrmis, Melanie; Grimwood, Keith; Bell, Scott C.; Whiley, David M.
- Abstract
<bold>Background: </bold>Pulmonary exacerbations in cystic fibrosis (CF) remain poorly understood and treatment is usually targeted at Pseudomonas aeruginosa. Within Australia a predominant shared P. aeruginosa strain (AUST-02) is associated with greater treatment needs. This single centre study assessed temporal shared strain population dynamics during and after antibiotic treatment of exacerbations.<bold>Methods: </bold>Sputum was collected from 12 adult patients with a history of chronic AUST-02 infection at four time-points during and after treatment of an exacerbation. Forty-eight P. aeruginosa isolates within each sample underwent AUST-02 allele-specific PCR and SNP-based strain genotyping.<bold>Results: </bold>Various commonly shared Australian strains (AUST-01, 0.1%; AUST-02, 54.3%; AUST-06, 36.6%; AUST-07, 4.6%; AUST-11, 4.3%) and two unique strains (0.1%) were identified from 45 sputum samples (2160 isolates). Based on within-patient relative abundance of strains, a "single-strain infection" (n = 7) or "mixed-strain infection" (n = 5) was assigned to each patient. A significant temporal variation in the P. aeruginosa population composition was found for those with mixed-strain infection (P < 0.001). Patients with mixed-strain infections had more long-term treatment requirements than those with single-strain infection. Moreover, despite both groups having similar lung function at study entry, patients with single-strain infection had greater improvement in FEV1% predicted following their exacerbation treatment (P = 0.02).<bold>Conclusion: </bold>Pulmonary exacerbations may reveal multiple, unrelated P. aeruginosa strains whose relative abundance with one another may change rapidly, in a sustained and unpredictable manner.
- Subjects
PSEUDOMONAS aeruginosa infections; CYSTIC fibrosis; PSEUDOMONAS aeruginosa; SPUTUM; GENOTYPES; ANTIBIOTICS; SPUTUM microbiology; DEMOGRAPHY; PSEUDOMONAS; PSEUDOMONAS diseases; RESPIRATORY infections; TIME; PHENOTYPES; VITAL capacity (Respiration); DISEASE progression; DISEASE complications
- Publication
BMC Pulmonary Medicine, 2017, Vol 17, p1
- ISSN
1471-2466
- Publication type
journal article
- DOI
10.1186/s12890-017-0482-7