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- Title
A new approach to dual-color two-photonmicroscopy with fluorescent proteins.
- Authors
Tillo, Shane E.; Hughes, Thomas E.; Makarov, Nikolay S.; Rebane, Aleks; Drobizhev, Mikhail
- Abstract
Background: Two-photon dual-color imaging of tissues and cells labeled with fluorescent proteins (FPs) is challenging because most two-photon microscopes only provide one laser excitation wavelength at a time. At present, methods for two-photon dual-color imaging are limited due to the requirement of large differences in Stokes shifts between the FPs used and their low two-photon absorption (2PA) efficiency. Results: Here we present a new method of dual-color two-photon microscopy that uses the simultaneous excitation of the lowest-energy electronic transition of a blue fluorescent protein and a higher-energy electronic transition of a red fluorescent protein. Conclusion: Our method does not require large differences in Stokes shifts and can be extended to a variety of FP pairs with larger 2PA efficiency and more optimal imaging properties.
- Subjects
MICROSCOPY; IMAGING systems; HEALTH care rationing; PROTEINS; WAVELENGTHS
- Publication
BMC Biotechnology, 2010, Vol 10, p6
- ISSN
1472-6750
- Publication type
Article
- DOI
10.1186/1472-6750-10-6