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- Title
Longan flower proanthocyanidins induce apoptosis in HT-29 colorectal carcinoma spheroids.
- Authors
Chung, Yuan-Chiang; Chiang, Hua-Che; Chang, Hsiang; Lin, Chih-Cheng; Lo, Li-Tsai; Wang, Ai-Yih; Chou, Kuo-Feng; Hsu, Chih-Ping
- Abstract
<bold>Aim Of Study: </bold>Proanthocyanidin-rich longan flower extract (LFP) has been previously shown to inhibit the proliferation and anchorage-independent growth in soft agar of two colorectal carcinoma (CRC) cells in vitro. In this report, we further examined the effects of LFP in a CRC spheroid model.<bold>Materials and Methods: </bold>A liquid-overlay assay employing HT-29 spheroids was used to evaluate the effects of LFP on cancer cell tumorigenesis, viability, and apoptosis. Associated effects on signaling path ways (epidermal growth factor receptor [EGFR], Akt) and apoptotic regulators were measured using Western blot.<bold>Results: </bold>Treatment with LFP up to 200 μg/ml inhibited tumor growth in a dose-dependent manner and induced prominent apoptosis as measured by annexin V staining. Cells treated with LFP showed decreased EGFR and Akt phosphorylation with decreased expression of B-cell lymphoma 2.<bold>Conclusion: </bold>The ability of LFP to induce apoptosis in CRC spheroids warrants further investigation of its composition and identification of tumor-active components.
- Subjects
LONGAN; APOPTOSIS; PROANTHOCYANIDINS; COLON cancer; CELL proliferation; CELL metabolism; ANTINEOPLASTIC agents; CELL physiology; CELLS; COLON tumors; EPIDERMAL growth factor; FLOWERS; PHOSPHORYLATION; PLANTS; RECTUM tumors; TRANSFERASES; PLANT extracts; CANCER cell culture; PHARMACODYNAMICS
- Publication
Journal of Cancer Research & Therapeutics, 2018, Vol 14, p388
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/0973-1482.176170