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- Title
The oxidized form of vitamin C, dehydroascorbic acid, regulates neuronal energy metabolism.
- Authors
Cisternas, Pedro; Silva ‐ Alvarez, Carmen; Martínez, Fernando; Fernandez, Emilio; Ferrada, Luciano; Oyarce, Karina; Salazar, Katterine; Bolaños, Juan P.; Nualart, Francisco
- Abstract
Vitamin C is an essential factor for neuronal function and survival, existing in two redox states, ascorbic acid ( AA), and its oxidized form, dehydroascorbic acid ( DHA). Here, we show uptake of both AA and DHA by primary cultures of rat brain cortical neurons. Moreover, we show that most intracellular AA was rapidly oxidized to DHA. Intracellular DHA induced a rapid and dramatic decrease in reduced glutathione that was immediately followed by a spontaneous recovery. This transient decrease in glutathione oxidation was preceded by an increase in the rate of glucose oxidation through the pentose phosphate pathway ( PPP), and a concomitant decrease in glucose oxidation through glycolysis. DHA stimulated the activity of glucose-6-phosphate dehydrogenase, the rate-limiting enzyme of the PPP. Furthermore, we found that DHA stimulated the rate of lactate uptake by neurons in a time- and dose-dependent manner. Thus, DHA is a novel modulator of neuronal energy metabolism by facilitating the utilization of glucose through the PPP for antioxidant purposes.
- Subjects
VITAMIN C; ENERGY metabolism regulation; NEURONS; BRAIN disease treatment; OXIDATION-reduction reaction; DOCOSAHEXAENOIC acid; LABORATORY rats
- Publication
Journal of Neurochemistry, 2014, Vol 129, Issue 4, p663
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/jnc.12663