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- Title
The Design of a Gene Chip for Functional Immunological Studies on a High-Quality Control Platform.
- Authors
WAUKAU, JILL; JAILWALA, PARTHAV; WANG, YOUMIN; KHOO, HUOY-JII; GHOSH, SOUMITRA; WANG, XUJING; HESSNER, MARTIN J.
- Abstract
A bstract: We have created an immunology-related microarray chip containing primarily known genes with well-studied functional properties. By looking at known genes rather than expressed sequence tags, we hope to gain a better understanding of immunological pathways and how they work. The immunology gene chip contains genes from the following functional categories: T cell genes; B cell genes; dendritic cell genes; chemokine and cytokine genes; apoptosis genes; cell cycle genes; cell interaction genes; general hematology and immunology genes; and adhesion genes. We have also developed a novel three-color cDNA array platform in which arrays are directly visualized before hybridization, which allows us to select only high-quality chips for our experiments. In an effort to provide quantitative quality control for each array element as well as the entire chip, we have developed Matarray, a software package for image processing and data acquisition. With Matarray, we have built a quantitative data filtering and normalization scheme that has proved to be more efficient than the existing methods. The list of immunology chip genes is available from the authors.
- Publication
Annals of the New York Academy of Sciences, 2003, Vol 1005, Issue 1, p284
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1288.044