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- Title
Flow cytometric evaluation of ki67 for the determination of malignancy grade in canine lymphoma.
- Authors
Poggi, A.; Miniscalco, B.; Morello, E.; Comazzi, S.; Gelain, M. E.; Aresu, L.; Riondato, F.
- Abstract
Ki67 is a nuclear antigen significantly correlated with degree of malignancy in human non-Hodgkin lymphomas. We wanted to assess the ability of flow cytometric evaluation of Ki67 index ( Ki67I) in differentiating the grade of malignancy in canine lymphomas. Ki67I was determined on lymph node aspirates of 90 immunophenotyped lymphomas classified according to the updated Kiel classification: 80 high grade (HG, 62 B cell and 18 T cell) and 10 low grade (LG, 3 B cell and 7 T cell) lymphomas. HG lymphomas showed significantly higher Ki67I compared with LG lymphomas ( P < 0.0001). A significant difference in HG lymphomas was detected between B- and T-immunophenotypes. Receiver operating characteristic ( ROC) curve highlighted a high accuracy of Ki67I in recognizing HG lymphomas [area under the curve ( AUC) = 99.4] and a cut-off value of 12.2% was established (sensitivity = 96.3% and specificity = 100%). Thus, we suggest the combination of Ki67I flow cytometric determination and immunophenotype as a reliable tool to classify canine lymphomas.
- Subjects
RETICULOENDOTHELIAL granulomas; RECEIVER operating characteristic curves; LYMPHOCYTES; ANTIGEN presenting cells; HUMORAL immunity
- Publication
Veterinary & Comparative Oncology, 2015, Vol 13, Issue 4, p475
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/vco.12078