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- Title
CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein.
- Authors
Tang, Lichun; Zeng, Yanting; Du, Hongzi; Lei, Ming; Liu, Jianqiao; Gong, Mengmeng; Peng, Jin; Zhang, Buxi; Zhao, Fang; Wang, Weihua; Li, Xiaowei
- Abstract
Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutations. However, whether this system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here we demonstrate that CRISPR/Cas9 is also effective as a gene-editing tool in human 2PN zygotes. By injection of Cas9 protein complexed with the appropriate sgRNAs and homology donors into one-cell human embryos, we demonstrated efficient homologous recombination-mediated correction of point mutations in HBB and G6PD. However, our results also reveal limitations of this correction procedure and highlight the need for further research.
- Subjects
CRISPRS; GENOME editing; ZYGOTES; GENETIC mutation; HUMAN embryo therapy; HOMOLOGY (Biology)
- Publication
Molecular Genetics & Genomics, 2017, Vol 292, Issue 3, p525
- ISSN
1617-4615
- Publication type
Article
- DOI
10.1007/s00438-017-1299-z