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- Title
Genetic diversity analysis in Vicia species using retrotransposon-based SSAP markers.
- Authors
Alberto Sanz; Susana Gonzalez; Naeem Syed; Maria Suso; Constantino Saldaña; Andrew Flavell
- Abstract
Abstract  Twelve different Ty1-copia and Ty3-gypsy group LTR retrotransposons were compared for their usefulness in SSAP marker development in two agriculturally important Vicia species. Three of the retrotransposons, PDR1, Tps19 and Tvf4, yielded useful SSAP marker systems in V. faba, and V. narbonensis. Another, Tvf1 was a good source of SSAP markers in V. narbonensis alone. The optimized SSAP marker systems were applied to the analysis of two diverse Vicia germplasm sets. Two hundred and two polymorphic Tvf1 SSAP markers were scored in 56 V. narbonensis samples and 196 polymorphic markers derived from the other three most useful retrotransposons were scored in a collection of 20 V. faba samples. The marker data were then used to construct phylogenetic trees. The trees for both species tend to show long-branch lengths, with rather little fine structure. Some V. narbonensis accessions cluster by geographical origin but many do not and a given geographical region is often represented by multiple diverse groups in the tree, suggesting a deep and ancient structure for the diversity of V. narbonensis that spans its current geographic range. The tree for the V. faba accessions also shows very limited clustering with geographical origin and no obvious correlation between diversity and morphology-based taxonomic groupings for the species.
- Subjects
FAVA bean; POLYMORPHIDAE; POLYMORPHIDA; GENETIC polymorphisms; PLANT species
- Publication
Molecular Genetics & Genomics, 2007, Vol 278, Issue 4, p433
- ISSN
1617-4615
- Publication type
Article
- DOI
10.1007/s00438-007-0261-x