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- Title
Simultaneous Determination of Decursin, Decursinol Angelate, Nodakenin, and Decursinol of Angelica gigas Nakai in Human Plasma by UHPLC-MS/MS: Application to Pharmacokinetic Study.
- Authors
Kim, Sook-Jin; Ko, Se-Mi; Choi, Eun-Jeong; Ham, Seong-Ho; Kwon, Young-Dal; Lee, Yong-Bok; Cho, Hea-Young; Marcotullio, Maria Carla
- Abstract
Coumarins in Cham-dang-gwi, the dried root of <italic>Angelica gigas</italic> Nakai (AGN), possess pharmacological effects on anemia, pain, infection, and articular rheumatism. The AGN root containes decursin (D), decursinol angelate (DA), nodakenin, and decursinol (DOH), a major metabolite of D and DA. The aim of this study was to develop a simultaneous determination method for these four coumarins in human plasma using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Chromatographic separation was performed on dual columns (Kinetex® C18 column and Capcell core C18 column) with mobile phase consisting of water and acetonitrile at a flow rate of 0.3 mL/min using gradient elution. Multiple reaction monitoring was operated in positive ion mode with precursors to product ion transition values of <italic>m</italic>/<italic>z</italic> 328.9→228.8, 328.9→228.9, 409.4→248.8, and 246.8→212.9 to measure D, DA, nodakenin, and DOH, respectively. Linear calibration curves were fitted over concentration range of 0.05–50 ng/mL for these four components, with correlation coefficient greater than 0.995. Inter- and intra-day accuracies were between 90.60% and 108.24%. These precisions were within 11.19% for all components. The established method was then applied to a pharmacokinetic study for the four coumarins after usual dosing in Korean subjects.
- Subjects
COUMARINS; BIOACTIVE compounds; PHARMACOLOGY; HERBAL medicine; LIQUID chromatography-mass spectrometry
- Publication
Molecules, 2018, Vol 23, Issue 5, p1019
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules23051019