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- Title
Simultaneous Determination of Four Tanshinones by UPLC-TQ/MS and Their Pharmacokinetic Application after Administration of Single Ethanol Extract of Danshen Combined withWater Extract in Normal and Adenine-Induced Chronic Renal Failure Rats.
- Authors
Hong-Die Cai; Shu-Lan Su; Yonghui Li; Zhenhua Zhu; Jianming Guo; Yue Zhu; Sheng Guo; Dawei Qian; Jinao Duan
- Abstract
Salvia miltiorrhiza, one of the major traditional Chinese medicines, is commonly used and the main active ingredients--tanshinones--possess the ability to improve renal function. In this paper, the UPLC-TQ/MS method of simultaneously determining four tanshinones--tanshinone IIA, dihydrotanshinone I, tanshinone I, and cryptotanshinone--was established and applied to assess the pharmacokinetics in normal and chronic renal failure (CRF) rat plasma. The pharmacokinetics of tanshinones in rats were studied after separately intragastric administration of Salvia miltiorrhiza ethanol extract (SMEE) (0.65 g/kg), SMEE (0.65 g/kg) combined with Salvia miltiorrhiza water extract (SMWE) (1.55 g/kg). The results showed Cmax and AUC0-t of tanshinone IIA, tanshinone I, cryptotanshinone reduced by 50%~80% and CLz/F increased by 2~4 times (p < 0.05) in model group after administrated with SMEE. Nevertheless, after intragastric administration of a combination of SMWE and SMEE, the Cmax and AUC0-t of four tanshinones were upregulated and CLz/F was downregulated, which undulated similarity from the model group to the normal group with compatibility of SMEE and SMWE. These results hinted that SMWE could improve the bioavailability of tanshinones in CRF rats, which provides scientific information for further exploration the mechanism of the combination of SMWE and SMEE and offers a reference for clinical administration of Salvia miltiorrhiza.
- Subjects
PHARMACOKINETICS; ETHANOL; SALVIA miltiorrhiza; PLANT extracts; CHRONIC kidney failure; LABORATORY rats
- Publication
Molecules, 2016, Vol 21, Issue 12, p1630
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules21121630