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- Title
Complete sequence of a viral nervous necrosis virus (NNV) isolated from red-spotted grouper ( Epinephelus akaara) in China.
- Authors
Liu, Hong; Teng, Yong; Zheng, Xiaocong; Wu, Yurong; Xie, Xiayang; He, Junqiang; Ye, Yiyou; Wu, Zhixin
- Abstract
A nodavirus isolated from red-spotted grouper ( Epinephelus akaara) larvae in China has been subjected to genome analysis. The full-length genome sequences of RNA1 and RNA2 were determined, and the 5′-non-coding region (NCR) and 3′NCR sequences were determined by 5′ rapid amplification of cDNA ends (RACE) and 3′RACE. RNA1 is 3,103 nt in length and contains a 982-amino-acid open reading frame (ORF) encoding protein A with a calculated molecular mass of 110.74 kDa. RNA2 is 1,433 nt long and contains a 338-amino-acid major ORF encoding coat protein with a calculated molecular mass of 37.059 kDa. Multiple alignment and phylogenetic analysis clearly supported including this virus in the species Redspotted grouper nervous necrosis virus, genus Betanodavirus, family Nodaviridae.
- Subjects
CHINA; NUCLEOTIDE sequence; EPINEPHELUS akaara; VIRUS diseases in fishes; OPEN reading frames (Genetics); PHYLOGENY; COAT proteins (Viruses); VIRUSES
- Publication
Archives of Virology, 2012, Vol 157, Issue 4, p777
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-011-1187-5