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- Title
The Effect of Hypothermia on Thyroxine Metabolism and Oxygen Consumption in the Isolated Perfused Rat Liver.
- Authors
Hassan, I.M.; Al-Ali, S.Y.; Hassan, M.; Sadek, S.; Clavel, M.
- Abstract
Isolated rat livers were perfused in vitro with Ham's F-10 perfusion medium at hypothermic (10°C) and normothermic (37 °C) temperatures. Thyroxine (T4) metabolism, oxygen utilization and hepatocyte fine structure were studied. Livers perfused at 10°C showed a significant reduction in hepatic T4 uptake (3.77 ± 0.65 μg/g) and triiodothyronine (T3) production (0.06 ± 0.03 μg/g) in comparison with livers perfused at 37°C (T4 uptake: 7.24 ± 2.38 μg/g, p < 0.0001; T3 released: 0.29 ± 0.09 μg/g, p < 0.0001). At this low temperature (10 °C), the livers utilize less oxygen (0.85 ± 0.06 μmol O2/min/g) than livers perfused at 37°C (2.2 ± 0.17 μmol O2/min/g). The livers perfused at 10°C remained intact at the ultrastructural level for 3 h, whilst at 37°C hepatocytes in the centrilobular zone showed changes in the nuclear chromatin and cell organelles. In conclusion, isolated rat livers perfused at 10°C showed significant reduction in oxygen utilization and hepatic thyroxine metabolism with intact morphological integrity, whilst perfusion at 37°C resulted in increased thyrosine metabolism but with marked fine structural changes in the hepatocyte organelles. Copyright © 1990 S. Karger AG, Basel
- Publication
Medical Principles & Practice, 1990, Vol 2, Issue 1, p47
- ISSN
1011-7571
- Publication type
Article
- DOI
10.1159/000157332