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- Title
Extraction-Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2.
- Authors
Carney, Patrick R.; Duellman, Tyler; Chan, Jia-Yi; Wells, Lauren; Tessmer, Michael; Frater-Rubsam, Leah; Zeller, Molly; Field, Mark; Speers, James; Tyrrell, Kelly; Thompson, Luke; Bondurant, Michael; Morin, Tami; Dagnon, Tamra; Goff, Brian; Runde, Corissa; Splinter-Bondurant, Sandra; Konsitzke, Charles; Kelly, Patrick; Bradfield, Christopher A.
- Abstract
The COVID-19 pandemic necessitated sensitive, fast, and inexpensive testing for the virus in 2020 prior to the widespread availability of vaccines. Early testing efforts were limited by bottlenecks on reagents, low-throughput testing options, and the slow return of test results. In this paper, we detail the testing pipeline we established at the University of Wisconsin-Madison for rapid, inexpensive, and sensitive surveillance testing for SARS-CoV-2, and we highlight the strengths of the platform that would allow it to be applied to other disease surveillance projects, SARS-CoV-2 variant testing, or future pandemics. This pipeline can be quickly established for further accreditation and clinical application.
- Subjects
COVID-19 pandemic; PUBLIC health; SARS-CoV-2; REVERSE transcriptase polymerase chain reaction; COVID-19 vaccines
- Publication
COVID, 2023, Vol 3, Issue 7, p1031
- ISSN
2673-8112
- Publication type
Article
- DOI
10.3390/covid3070075