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- Title
Biodegradation of 3-nitrotoluene by Rhodococcus sp. strain ZWL3NT.
- Authors
Tian, Xiao-Jun; Liu, Xiao-Yang; Liu, Hong; Wang, Shu-Jun; Zhou, Ning-Yi
- Abstract
A pure bacterial culture was isolated by its ability to utilize 3-nitrotoluene (3NT) as the sole source of carbon, nitrogen, and energy for growth. Analysis of its 16S rRNA gene showed that the organism (strain ZWL3NT) belongs to the genus Rhodococcus. A rapid disappearance of 3NT with concomitant release of nitrite was observed when strain ZWL3NT was grown on 3NT. The isolate also grew on 2-nitrotoluene, 3-methylcatechol and catechol. Two metabolites, 3-methylcatechol and 2-methyl- cis,cis-muconate, in the reaction mixture were detected after incubation of cells of strain ZWL3NT with 3NT. Enzyme assays showed the presence of both catechol 1,2-dioxygenase and catechol 2,3-dioxygenase in strain ZWL3NT. In addition, a catechol degradation gene cluster ( catRABC cluster) for catechol ortho-cleavage pathway was cloned from this strain and cell extracts of Escherichia coli expressing CatA and CatB exhibited catechol 1,2-dioxygenase activity and cis, cis-muconate cycloisomerase activity, respectively. These experimental evidences suggest a novel pathway for 3NT degradation with 3-methylcatechol as a key metabolite by Rhodococcus sp. strain ZWL3NT.
- Subjects
BIODEGRADATION; NITROTOLUENE; BACTERIAL cultures; RHODOCOCCUS; GUAIACOL; CATECHOL
- Publication
Applied Microbiology & Biotechnology, 2013, Vol 97, Issue 20, p9217
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-012-4619-6