We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Novel aerobic benzene degrading microorganisms identified in three soils by stable isotope probing.
- Authors
Shuguang Xie; Weimin Sun; Chunling Luo; Cupples, Alison M.
- Abstract
The remediation of benzene contaminated groundwater often involves biodegradation and although the mechanisms of aerobic benzene biodegradation in laboratory cultures have been well studied, less is known about the microorganisms responsible for benzene degradation in mixed culture samples or at contaminated sites. To address this knowledge gap, DNA based stable isotope probing (SIP) was utilized to identify active benzene degraders in microcosms constructed with soil from three sources (a contaminated site and two agricultural sites). For this, replicate microcosms were amended with either labeled (C) or unlabeled benzene and the extracted DNA samples were ultracentrifuged, fractioned and subject to terminal restriction fragment length polymorphism (TRFLP). The dominant benzene degraders (responsible for C uptake) were determined by comparing relative abundance of TRFLP phylotypes in heavy fractions of labeled benzene (C) amended samples to the controls (from unlabeled benzene amended samples). Two phylotypes (a Polaromonas sp. and an Acidobacterium) were the major benzene degraders in the microcosms constructed from the contaminated site soil, whereas one phylotype incorporated the majority of the benzene-derived C in each of the agricultural soils ('candidate' phylum TM7 and an unclassified Sphingomonadaceae).
- Subjects
BENZENE biodegradation; AEROBIC bacteria; AROMATIC compounds; BIODEGRADATION; MICROBIOLOGY; BIOCHEMISTRY
- Publication
Biodegradation, 2011, Vol 22, Issue 1, p71
- ISSN
0923-9820
- Publication type
Article
- DOI
10.1007/s10532-010-9377-5