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- Title
Validation of [<sup>123</sup>I]Β-CIT SPECT to Assess Serotonin Transporters In Vivo in Humans: a Double-Blind, Placebo-Controlled, Crossover Study with the Selective Serotonin Reuptake Inhibitor Citalopram.
- Authors
De Win, Maartje ML; Habraken, Jan BA; Reneman, Liesbeth; van Den Brink, Wim; den Heeten, Gerard J.; Booij, Jan
- Abstract
Disturbances in the serotonin (5-HT) system are associated with various neuropsychiatric disorders. The 5-HT system can be studied in vivo by measuring 5-HT transporter (SERT) densities using 123iodine-labeled 2β-carbomethoxy-3β(4-iodophenyl)tropane ([123I]β-CIT) and single photon emission computed tomography (SPECT). Validation of this technique is important because [123I]β-CIT does not bind selectively to SERTs. Some studies have validated this technique in vivo in the human brain in SERT-rich areas, but the technique has not been validated yet in SERT-low cortical areas. The aim of this study was to further validate [123I]β-CIT SPECT in assessing SERTs in vivo in humans in both SERT-rich and SERT-low areas. A double-blind, placebo-controlled, crossover design was used with the selective 5-HT reuptake inhibitor (SSRI) citalopram. Six male subjects underwent two [123I]β-CIT SPECT sessions: one after pretreatment with citalopram and one after placebo. Scans were acquired 4 h and 22-27 h p.i., and both region-of-interest and voxel-by-voxel analyses were performed. Citalopram reduced [123I]β-CIT binding ratios in SERT-rich midbrain and (hypo)thalamus. Binding ratios were also Iower after citalopram in SERT-low cortical areas, but statistical significance was only reached in several cortical areas using voxel-by-voxel analysis. In addition, citalopram increased binding ratios in the DAT-rich striatum and increased absolute uptake in the cerebellum. The results show that [123I]β-CIT SPECT is a valid technique to study SERT binding in vivo in human brain in SERT-rich areas. Although we provide some evidence that [123I]β-CIT SPECT may be used to measure SERTs in SERT-Iow cortical areas, these measurements must be interpreted with caution.
- Subjects
NEUROTRANSMITTERS; TOMOGRAPHY; SEROTONIN; MEDICAL radiography; THALAMUS; PLACEBOS
- Publication
Neuropsychopharmacology, 2005, Vol 30, Issue 5, p996
- ISSN
0893-133X
- Publication type
Article
- DOI
10.1038/sj.npp.1300683