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- Title
Isolation and expression analysis of a poriferanAntp-classBar-/Bsh-like homeobox gene.
- Authors
Hill, April; Tetrault, Jeff; Hill, Malcolm
- Abstract
A novel non-HoxAntp-class gene (BarBsh-Hb) was isolated from the marine spongeHalichondriasp. This gene shares high sequence identity with eumetazoan genes from theBshandBargene families and can be distinguished from other non-Hox Antp-class genes by diagnostic residues. We also present an alignment of all known (full-length) poriferan non-HoxAntp-class genes. Maximum likelihood methods were employed to estimate phylogenetic relationships among non-Hoxgenes andBarBsh-Hb. We employed RT-PCR techniques to look at expression across different developmental stages (larval to rhagon).BarBsh-Hbproduct was present in newly released larvae, but expression was not detected 8-16 h post-release. Expression ofBarBsh-Hbwas detected in later-stage (>16 h post-release), free-swimming larvae until they settled and attached to the substratum, after which expression was down-regulated. In a separate set of experiments, low levels of expression were observed in normal adult tissue and disaggregated adult tissue, butBarBsh-Hbexpression increased during tissue re-aggregation. These data increase the number of non-Hoxhomeobox genes identified in sponges and provide evidence of regulation of this non-Hoxgene during sponge development. While theBarandBshgenes play important roles in the development of nervous tissue-especially visual systems-in metazoans, the specific role(s)BarBsh-Hbplay(s) in sponge development is unclear and deserves greater attention.
- Subjects
SPONGES (Invertebrates); DEVELOPMENTAL biology; GENES; LARVAE; TISSUES; MARINE invertebrates
- Publication
Development Genes & Evolution, 2004, Vol 214, Issue 10, p515
- ISSN
0949-944X
- Publication type
Article
- DOI
10.1007/s00427-004-0431-3