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- Title
Priming Wharton Jelly's Mesenchymal Stem Cells Enhance Immunosuppressive Properties and Angiogenic Potential.
- Authors
Yu Ling Tan; Sue Ping Eng; Min Hwei Ng
- Abstract
Introduction: Mesenchymal stem cells (MSCs) have shown promising in cardiovascular disease over the past decade, whereby MSCs have been demonstrated to inhibit myocardial fibrosis, promote neovascularization, and induce macrophage polarization. Interestingly, different culture conditions of MSCs influence the phenotype and secretion of MSCs and subsequently alter the MSCs' functionality. Priming with cytokines or growth factors or hypoxia preconditioned has been shown to affect the gene expression and protein secretion of MSCs. Thus, this study evaluates the effects of the priming conditions for empowering the MSCs in terms of immunosuppressive properties and angiogenic potential. Methods: WJ-MSCs isolated from umbilical cords were primed with interferon-γ (IFN-γ) and hypoxia preconditioned. The effects of these priming conditions on the RNA expression of WJ-MSCs were evaluated. Results: mRNA levels of TGF-β, PGE2 and IDO were significantly increased in the IFN-γ primed WJ-MSCs group, suggesting that IFN-γ mediated the immunosuppressive properties of WJ-MSCs. Moreover, the mRNA levels of VEGF and bFGF were also significantly increased in the hypoxia preconditioned WJ-MSCs group, which suggests that the angiogenic potential of WJ-MSCs can be enhanced under hypoxic conditions. Conclusion: The present study suggested that IFN-γ and hypoxic preconditioned WJ-MSCs may possess greater immunosuppressive and angiogenic potential, enhancing their therapeutic efficacy in clinical translation. The preconditioned MSCs could potentially be administered to ameliorate the progression of myocardial infarction by suppressing inflammation and promoting revascularization in the peri-infarcted tissues. However, further analysis such as macrophage polarization and PBMC proliferation co-cultured with primed WJ-MSCs will be required to support this observation before conducting a proof-of-concept study in the myocardial infarction model.
- Subjects
MESENCHYMAL stem cells; MYOCARDIAL infarction; GROWTH factors; JELLY; PROTEIN expression
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p12
- ISSN
1675-8544
- Publication type
Article