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- Title
Identification and functional analysis of the novel ORF6 protein of porcine circovirus type 2 <italic>in vitro</italic>.
- Authors
Li, Dongge; Wang, Jing; Xu, Shangen; Cai, Shanxia; Ao, Chaojie; Fang, Liurong; Xiao, Shaobo; Chen, Huanchun; Jiang, Yunbo
- Abstract
In the present study, the function of a novel ORF6 gene in the PCV2 genome was determined and functionally analyzed <italic>in vitro</italic>. ORF6 expression was demonstrated by indirect immunofluorescence in PCV2-infected cells. The antibody against ORF6 was detected in PCV2-infected pigs. The start codon of ORF6 was mutated and an infectious clone was used to create an ORF6-deficient mutant virus. Viral DNA replication curves and immunofluorescence analysis indicated that ORF6 is unnecessary for viral replication and ORF6 deletion reduces viral DNA replication in PK-15 cells. The activities of caspases 3 and 8 in ORF6-deficient virus-infected cells were significantly different from those in wild-type virus-infected cells. The ORF6 protein can increase the expression of IFN-β, TNF-α, IL-1b, IL-10, and IL-12p40. These results demonstrated that the newly discovered ORF6 protein may be involved in caspases regulation and the expression of multiple cytokines in PCV2-infected cells. The functions of this gene in viral pathogenesis remain to be further elucidated.
- Publication
Veterinary Research Communications, 2018, Vol 42, Issue 1, p1
- ISSN
0165-7380
- Publication type
Article
- DOI
10.1007/s11259-017-9702-0