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- Title
Multiscale measurement of cardiac energetics.
- Authors
Goo, Soyeon; Pham, Toan; Han, Jun‐Chiew; Nielsen, Poul; Taberner, Andrew; Hickey, Anthony; Loiselle, Denis
- Abstract
Herein we describe our laboratories' experimental methods for interrogating cardiac energetics at the organ (whole heart), tissue (trabecula) and perforated fibre (mitochondrial) levels., In whole heart and trabecula experiments, we focus on measuring pressure-volume (force-length) work and oxygen consumption (heat production) from which mechanical efficiency is derived. In both preparations (i.e. across scales differing by three orders of magnitude) we find efficiency values of 10%-15%., Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron leak and reactive oxygen species production, the latter two of which index energy transfer inefficiencies.
- Subjects
HEART physiology; CARDIAC research; HEART beat; FLUX (Energy); OXYGEN consumption; MITOCHONDRIAL membranes
- Publication
Clinical & Experimental Pharmacology & Physiology, 2013, Vol 40, Issue 9, p671
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/1440-1681.12139