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- Title
Protection induced by vaccinating sheep with a thiol-binding extract ofHaemonchus contortusmembranes is associated with its protease components.
- Authors
Knox, D. P.; Smith, S. K.; Redmond, D. L.; Smith, W. D.
- Abstract
Previous work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adultHaemonchus contortususing thiol sepharose affinity chromatography confers substantial protection against a single challenge infection. TSBP comprised proteases and other proteins. Here, TSBP were further fractionated using anion-exchange chromatography and fractions pooled on the basis of containing (1) protease activity, (2) a prominent non-protease peptide and (3) material which did not bind to the column. A protection trial showed that only the protease-enriched material conferred protective immunity and this was comparable to that observed in a TSBP-immunized positive control group. Immunization stimulated a marked IgG response with the IgG2 isotype predominating.
- Subjects
CHROMATOGRAPHIC analysis; GEL permeation chromatography; PROTEOLYTIC enzymes; BIOMOLECULES; IMMUNIZATION; PREVENTIVE medicine
- Publication
Parasite Immunology, 2005, Vol 27, Issue 4, p121
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1111/j.1365-3024.2005.00750.x