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- Title
Correlation between the Density of Acipenser sinensis and Its Environmental DNA.
- Authors
Wang, Xiaojing; Feng, Guangpeng; Zhu, Jiazhi; Jiang, Wei
- Abstract
Simple Summary: The objective of this study was to investigate the correlation between juvenile Acipenser sinensis density and the eDNA content in a controlled laboratory aquarium environment. The results obtained from fluorescent quantitative PCR indicated an overall increasing trend in the eDNA content, which tended to stabilize after three days. Furthermore, a significant positive linear correlation was observed between the density of juvenile A. sinensis and eDNA content at different time points (R2 = 0.768~0.986). When the juvenile A. sinensis were removed at a density of 0.114 ind./L, there was a negative correlation between eDNA content and time. Based on AIC and BIC analyses, a power function model was employed to illustrate the degradation relationship between eDNA content and time. Since the construction of the Gezhouba Dam, Chinese sturgeon (Acipenser sinensis) numbers have gradually declined, rendering this species critically endangered according to the International Union for the Conservation of Nature. Environmental DNA (eDNA) technology plays an important role in monitoring the abundance of aquatic organisms. Species density and biomass have been proven to be estimable by researchers, but the level of accuracy depends on the specific species and ecosystem. In this study, juvenile A. sinensis, an endangered fish, were selected as the research target. Under controlled laboratory conditions in an aquarium, one, two, four, six, and eight juvenile A. sinensis were cultured in five fish tanks, respectively. Water samples were filtered at eight different time points for eDNA content analysis. Additionally, eDNA yield was tested at six different time points after a 0.114 ind./L density of A. sinensis was removed, and the employed degradation model was screened using the Akaike information criterion (AIC) and the Bayesian information criterion (BIC). The results showed that eDNA content remained stable after 3 days and exhibited a significant positive linear correlation with the density of A. sinensis (R2 = 0.768~0.986). Furthermore, eDNA content was negatively correlated with the 3-day period after the removal of A. sinensis. The power function had the smallest AIC and BIC values, indicating better fitting performance. This study lays a momentous foundation for the application of eDNA for monitoring juvenile A. sinensis in the Yangtze Estuary and reveals the applicability of eDNA as a useful tool for assessing fish density/biomass in natural environments.
- Subjects
ACIPENSER; NATURE conservation; RARE fishes; AKAIKE information criterion; FISH farming; WATER sampling; DENSITY
- Publication
Biology (2079-7737), 2024, Vol 13, Issue 1, p19
- ISSN
2079-7737
- Publication type
Article
- DOI
10.3390/biology13010019