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- Title
Multiplex Real-Time Reverse Transcription PCR for Influenza A Virus, Influenza B Virus, and Severe Acute Respiratory Syndrome Coronavirus 2.
- Authors
Bo Shu; Kirby, Marie K.; Davis, William G.; Warnes, Christine; Liddell, Jimma; Ji Liu; Kai-Hui Wu; Hassell, Norman; Benitez, Alvaro J.; Wilson, Malania M.; Keller, Matthew W.; Rambo-Martin, Benjamin L.; Camara, Yamundow; Winter, Jörn; Kondor, Rebecca J.; Bin Zhou; Spies, Stacey; Rose, Laura E.; Winchell, Jonas M.; Limbago, Brandi M.
- Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019, and the outbreak rapidly evolved into the current coronavirus disease pandemic. SARS-CoV-2 is a respiratory virus that causes symptoms similar to those caused by influenza A and B viruses. On July 2, 2020, the US Food and Drug Administration granted emergency use authorization for in vitro diagnostic use of the Influenza SARS-CoV-2 Multiplex Assay. This assay detects influenza A virus at 102.0, influenza B virus at 102.2, and SARS-CoV-2 at 100.3 50% tissue culture or egg infectious dose, or as few as 5 RNA copies/reaction. The simultaneous detection and differentiation of these 3 major pathogens increases overall testing capacity, conserves resources, identifies co-infections, and enables efficient surveillance of influenza viruses and SARS-CoV-2.
- Subjects
INFLUENZA B virus; INFLUENZA viruses; INFLUENZA A virus; COVID-19; INFLUENZA; COVID-19 pandemic
- Publication
Emerging Infectious Diseases, 2021, Vol 27, Issue 7, p1821
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2707.210462