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- Title
<italic>Flos Magnoliae</italic> Inhibits Chloride Secretion via ANO1 Inhibition in Calu-3 Cells.
- Authors
Kim, Hyun Jong; Nam, Yu Ran; Nam, Joo Hyun
- Abstract
Flos Magnoliae (FM, Chinese name: Xin-yi) is an oriental medicinal herb commonly used for symptomatic relief from allergic rhinitis, sinusitis, and headache, including in traditional Chinese and Korean medicine formulations. FM inhibits histamine release from mast cells and cytokine secretion from T cells. However, the mechanism of action of FM on the anoctamin-1 (ANO1) ion channel, which is responsible for nasal hypersecretion in allergic rhinitis, has not been elucidated. Therefore, in this study, we investigated the effect of a 30% ethanolic extract of FM (FMEtOH) and its chemical constituents on ANO1 activity. We used high-performance liquid chromatography analysis to identify five major chemical constituents of FMEtOH: vanillic acid, tiliroside, eudesmin, magnolin, and fargesin. Using a conventional whole-cell patch clamp method, we found that FMEtOH (30, 100, and 300 μ g/mL) and its chemical constituent tiliroside inhibited ANO1 activity in ANO1-overexpressing HEK293T cells. In addition, we found that the treatment of the airway epithelial cell line Calu-3 with interleukin 4 significantly increased Ca 2 + activated Cl − current (ICaCC), but not cystic fibrosis transmembrane conductance regulator (CFTR)-mediated chloride current (ICFTR). FMEtOH and tiliroside specifically inhibited ICaCC. Thus, in this study, we identified a novel mechanism underlying the alleviation of allergic rhinitis by FMEtOH. Our results indicate that FMEtOH and its chemical constituent tiliroside are promising and potent agents for the prevention and treatment of allergic rhinitis.
- Subjects
BIOLOGICAL transport; CELL lines; CHLORIDES; CYTOLOGICAL techniques; SEASONAL variations of diseases; DOSE-effect relationship in pharmacology; ETHANOL; GENETIC techniques; ALLERGIC rhinitis; HERBAL medicine; HIGH performance liquid chromatography; INTERLEUKINS; CHINESE medicine; MEMBRANE proteins; MOLECULAR structure; RESEARCH funding; STATISTICS; T-test (Statistics); PHYTOCHEMICALS; DATA analysis; DATA analysis software; ONE-way analysis of variance; CHEMICAL inhibitors
- Publication
American Journal of Chinese Medicine, 2018, Vol 46, Issue 5, p1079
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X18500568