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- Title
Genistein Suppresses the Isoproterenol-Treated H9c2 Cardiomyoblast Cell Apoptosis Associated with P-38, Erk1/2, JNK, and κ Signaling Protein Activation.
- Authors
Hu, Wei-Syun; Lin, Yueh-Min; Ho, Tsung-Jung; Chen, Ray-Jade; Li, Yi-Hui; Tsai, Fuu-Jen; Tsai, Chang-Hai; Day, Cecilia Hsuan; Chen, Tung-Sheng; Huang, Chih-Yang
- Abstract
Heart disease (HD) is associated with estrogen and therefore gender and menopausal status. In addition, clinical evidence shows that increased serum norepinephrine is found in patients with HD. Therefore, this study aimed to investigate the cardio-protective effect of genistein, a selective estrogen receptor modulator (SERM) from soy bean extract, in H9c2 cardiomyoblast cells treated with isoproterenol (ISO), a norepinephrine analog. In this in vitro model, image data and results from western blotting shown that ISO treatment was capable of inducing cellular apoptosis, especially the mitochondrial dependent pathway. Treatment of genistein could suppress the expression of mitochondrial pro-apoptotic proteins including Bad, caspase-8, caspase-9, and caspase-3 in H9c2 treated with ISO. By contrast, several survival proteins were expressed in H9c2 treated with genistein, such as phosphor (p)-Akt, p-Bad, and p-Erk1/2. Furthermore, we confirmed that the protective role of genistein was partially mediated through the expression of Erk1/2, Akt, and κ proteins by adding several pathway inhibitors. These in vitro data suggest that genistein may be a safe and natural SERM alternative to hormone therapy in cardio-protection.
- Subjects
APOPTOSIS; CELL lines; CELLULAR signal transduction; HEART; ISOPROTERENOL; RESEARCH funding; WESTERN immunoblotting; PLANT extracts; GENISTEIN; IN vitro studies
- Publication
American Journal of Chinese Medicine, 2013, Vol 41, Issue 5, p1125
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X13500766