We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Preventive Effects of β-Thujaplicin Against UVB-Induced MMP-1 and MMP-3 mRNA Expressions in Skin Fibroblasts.
- Authors
Cherng, Jong Yuh; Chen, Li Yin; Shih, Mei Fen
- Abstract
Solar UV radiation damages human skin by affecting skin tone and resiliency and leads to premature aging (photoaging). The skin damage is caused by the activation of the AP-1 transcription factor, which increases matrix metalloproteinase (MMP) expression and collagen degradation. An increase of interleukin (IL)-6 is also correlated with the activation of MMP-1 expression. β-thujaplicin has shown both acaricidal and antimicrobial activities. Also, β-thujaplicin has been shown to be protective against apoptosis due to the oxidative effects of UV irradiation. However, the effect of β-thujaplicin on UVB-induced MMPs had not been investigated. In this study, after UVB exposure, MMP-1 and IL-6 production in human skin fibroblasts was examined in the presence of β-thujaplicin, vitamin C, and vitamin E. The expression of MMP-1, MMP-3, tissue inhibitor of metalloproteinase (TIMP-1, TIMP-3) and procollagen mRNA was also investigated. Results showed that UVB-induced MMP-1 production was suppressed by the β-thujaplicin treatment in a dose-dependent manner, but not by vitamin C and vitamin E. β-thujaplicin also prevented the up-regulation of MMP-1 and MMP-3 mRNA. Moreover, the UVB-suppressed procollagen gene expression was restored to normal by β-thujaplicin. Neither UVB nor β-thujaplicin affected the mRNA expression of TIMP-1 and TIMP-3. The IL-6 production induced by UVB was lower in β-thujaplicin treated fibroblasts than in the controls. In conclusion, this study shows the capability of β-thujaplicin in preventing MMP-1 production due to UVB irradiation via inhibition of MMP gene expression. Importantly, the UVB-suppressed procollagen gene expression can be restored to normal by β-thujaplicin. These findings indicate that β-thujaplicin is a promising and potent agent to inhibit UVB-induced MMP-1 and MMP-3 gene expression in skin fibroblasts.
- Subjects
ANTIOXIDANTS; BARK; DENSITOMETRY; FIBROBLASTS; GENE expression; MOLECULAR structure; PROTEOLYTIC enzymes; SKIN; SKIN aging; STATISTICAL hypothesis testing; T-test (Statistics); ULTRAVIOLET radiation; PLANT extracts; DATA analysis software; DESCRIPTIVE statistics
- Publication
American Journal of Chinese Medicine, 2012, Vol 40, Issue 2, p387
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X12500309