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- Title
Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain.
- Authors
Xinsheng Liu; Chun-Ming Lin; Annamalai, Thavamathi; Xiang Gao; Zhongyan Lu; Esseili, Malak A.; Kwonil Jung; El-Tholoth, Mohamed; Saif, Linda J.; Qiuhong Wang
- Abstract
The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD50) of the virus pool was determined as 7.35 log10 PDD50/mL, similar to the cell culture infectious titer, 7.75 log10 plaque-forming units (PFU)/mL. 100 PDD50 caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a PEDV-naive sow, whereas 1000 and 10 000 PDD50 did not cause diarrhea in piglets derived from two PEDV-field exposed-recovered sows. This information is important for future PEDV challenge studies and validation of PEDV vaccines.
- Subjects
VIRUS virulence; PORCINE epidemic diarrhea virus; COLOSTRUM; DIARRHEA; SUCKLING in animals; PIGLET physiology; PHYSIOLOGY; SWINE
- Publication
Veterinary Research, 2015, Vol 46, Issue 1, p1
- ISSN
0928-4249
- Publication type
Article
- DOI
10.1186/s13567-015-0249-1