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- Title
Oral administration of a select mixture of Bacillus probiotics generates Tr1 cells in weaned F4ab/acR<sup>-</sup> pigs challenged with an F4<sup>+</sup> ETEC/VTEC/EPEC strain.
- Authors
Dong Zhou; Yao-Hong Zhu; Wei Zhang; Meng-Ling Wang; Wen-Yi Fan; Dan Song; Gui-Yan Yang; Bent Borg Jensen; Jiu-Feng Wang
- Abstract
Although breeding of F4 receptor-negative (F4R -) pigs may prevent post-weaning diarrhea, the underlying immunity is poorly understood. Here, various doses of a Bacillus licheniformis and Bacillus subtilis mixture (BLS-mix) were orally administered to F4ab/acR - pigs for 1 week before F4 (K88) - positive ETEC/VTEC/EPEC challenge. Administration of BLS-mix increased the percentage of Foxp3 - IL-10 + T cells but not of Foxp3 + IL-10 + regulatory T (Treg) cells among peripheral blood CD4 + T cells. A low dose of BLS-mix feeding resulted in increased the expression of IL-6, TNF-α, IL-10, and the transcription factors Foxp3 and T-bet mRNAs in the jejunum. Administration of either a low or high dose BLS-mix also led to an increase in the percentage of CD4 + Foxp3 + Treg cells among intraepithelial lymphocytes and CD4 + IL-10 + T cells in the small intestinal Peyer's patches and the lamina propria of F4ab/acR - pigs following F4 + ETEC/VTEC/EPEC challenge. The increased number of IL-10-producing CD4 + T cells was attributed to an increase in the proportion of Foxp3 - IL-10 + Treg cells rather than Foxp3 + IL-10 + Treg cells. Our data indicate that oral administration of BLS-mix to newly weaned F4ab/acR - pigs ameliorates enteritis in an F4 + ETEC/VTEC/EPEC model; however, induction of IL-10-producing Foxp3 - Treg cells by BLS-mix administration cannot account for the protection of newly weaned F4ab/acR - pigs from F4 + ETEC/VTEC/EPEC infection, and that excessive generation of CD4 + IL-10 + T cells following consumption of BLS-mix during episodes of intestinal inflammation that is caused by enteric pathogens might prohibit clearance of the pathogen. Select probiotic mixtures may allow for tailoring strategies to prevent infectious diseases.
- Subjects
ESCHERICHIA coli infections in animals; ANIMAL weaning; SWINE disease prevention; DRUG administration; ORAL drug administration; PROBIOTICS; BACILLUS (Bacteria)
- Publication
Veterinary Research, 2015, Vol 46, Issue 1, p1
- ISSN
0928-4249
- Publication type
Article
- DOI
10.1186/s13567-015-0223-y