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- Title
<i>In Vitro</i> Ceramic Scaffold Mineralization: Comparison Between Histological and Micro-Computed Tomographical Analysis.
- Authors
Thimm, Benjamin W.; Wechsler, Oliver; Bohner, Marc; Müller, Ralph; Hofmann, Sandra
- Abstract
The porous structure of beta-tricalcium phosphate (β-TCP) scaffolds was assessed by conventional histomorphometry and micro-computed tomography (micro-CT) to evaluate the substitutability of time-consuming histomorphometry by rapid micro-CT. Extracellular matrix mineralization on human mesenchymal stem cell seeded β-TCP scaffolds was scanned by means of micro-CT after 6 weeks in cultivation and evaluated morphometrically. For the histomorphometric analysis, undecalcified sections were prepared in the mediosagittal plane of the cylindrical tissue-engineered constructs. The sections were scanned at a nominal resolution of 8 μm and stained with von Kossa and Toluidine Blue. Pores were analyzed with both methods for morphometrical parameters such as horizontal/vertical diameter and pore/mineralized tissue area. Results showed highly significant correlations between histomorphometry and micro-CT for pore horizontal length ( r = 0.95), pore vertical length ( r = 0.96), pore area ( r = 0.97), and mineralized tissue area ( r = 0.82). Mean percentage differences between histomorphometry and micro-CT measurements ranged from 1.4% (pore vertical diameter) to 14.0% (area of mineralized tissue). With its high image precision, micro-CT qualifies as an additional tool for endpoint evaluation measurements of mineralized tissue development within tissue-engineered constructs also in ceramic scaffolds.
- Subjects
TISSUE scaffolds; BIOMINERALIZATION; COMPUTED tomography; CERAMIC materials; POROSITY; CALCIUM phosphate; EXTRACELLULAR matrix
- Publication
Annals of Biomedical Engineering, 2013, Vol 41, Issue 12, p2666
- ISSN
0090-6964
- Publication type
Article
- DOI
10.1007/s10439-013-0877-4