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- Title
Evolution of Gene Expression after Gene Amplification.
- Authors
Garcia, Nelson; Wei Zhang; Yongrui Wu; Messing, Joachim
- Abstract
We took a rather unique approach to investigate the conservation of gene expression of prolamin storage protein genes across two different subfamilies of the Poaceae. We took advantageof oat plants carrying single maize chromosomes in different cultivars, called oat-maize addition (OMA) lines, which permitted us to determine whether regulation of gene expression was conserved between the two species. We found that -zeins are expressed in OMA7.06, which carries maize chromosome 7 even in the absence of the trans-actingmaizeprolamin-box-bindingfactor (PBF), which regulates their expression. This is likelybecause oat PBF can substitute for the function of maize PBF as shownin our transient expression data, using a B-zeinpromoter fused to green fluorescent protein (GFP). Despite this conservation, the younger, recently amplified prolamin genes in maize, absent in oat, are not expressed in the corresponding OMAs. However, maize can express the oldest prolamin gene, the wheat high-molecular weight glutenin Dx5 gene, even when maize Pbf is knocked down (through PbfRNAi), and/or another maize transcription factor, Opaque-2 (O2) is knocked out (in maize o2 mutant). Therefore, older genes are conserved in their regulation, whereas younger ones diverged during evolution and eventually acquired a new repertoire of suitable transcriptional activators.
- Subjects
GENE expression; GENE amplification; BIOLOGICAL evolution; GREEN fluorescent protein; MOLECULAR weights
- Publication
Genome Biology & Evolution, 2015, Vol 7, Issue 5, p1303
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evv075