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- Title
Protective immunity against Trichinella spiralis infection induced by TsNd vaccine in mice.
- Authors
Pei Liu; Jing Cui; Ruo Dan Liu; Min Wang; Peng Jiang; Li Na Liu; Shao Rong Long; Ling Ge Li; Shuai Bing Zhang; Xin Zhuo Zhang; Zhong Quan Wang
- Abstract
Background: We have previously reported that Trichinella spiralis Nudix hydrolase (TsNd) bound to intestinal epithelial cells (IECs), and vaccination of mice with recombinant TsNd protein (rTsNd) produced a partial protective immunity. The aim of this study was to investigate the immune protection induced by TsNd DNA vaccine. Methods: The full-length cDNA sequence of TsNd gene was cloned into pcDNA3.1 and used to immunize BALB/c mice by intramuscular injection. Transcription and expression of TsNd were detected by RT-PCR and IFT. The levels of specific IgA, IgG, IgG1 and IgG2a, and cytokines were assayed by ELISA at weeks 0, 6 and 8 post-immunization. The immune protection of TsNd DNA vaccine against challenge infection was investigated. Results: Immunization of mice with TsNd DNA elicited a systemic Th1/Th2 immune response and a local mucosal IgA response. The in vitro transcription and expression of TsNd gene was observed at all developmental stages of T. spiralis (ML, IIL, AW and NBL). Anti-rTsNd IgG levels were increased after immunization and levels of IgG1 were obviously higher than that of IgG2a. Intestinal specific IgA levels of immunized mice were significantly higher than those of vector and PBS control mice. Cytokine profiling also showed a significant increase in Th1 (IFN-γ, IL-2) and Th2 (IL-4, 10) responses in splenocytes of immunized mice on stimulation with rTsNd. Vaccination of mice with pcDNA3.1-TsNd displayed a 40.44% reduction in adult worms and a 53.9% reduction in larval burden. Conclusions: TsNd DNA induced a mixed Th1/Th2 immune response and partial protection against T. spiralis infection in mice.
- Subjects
TRICHINELLA spiralis; RECOMBINANT antibodies; LABORATORY mice; TRICHINELLA; EPITHELIAL cells; IMMUNIZATION; CYTOKINES
- Publication
Parasites & Vectors, 2015, Vol 8, Issue 1, p1
- ISSN
1756-3305
- Publication type
Article
- DOI
10.1186/s13071-015-0791-8