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- Title
Inhibitory Effects of the Methylene Chloride Fraction of JP05 on the Production of Inflammatory Mediators in LPS-activated BV2 Microglia.
- Authors
Jung, Hyo; Oh, Tae; Jung, Jin; Lee, Je-Hyun; Shin, Gil; Park, Yong-Ki
- Abstract
Excessive production of inflammatory mediators such as nitric oxide (NO) and proinflammatory cytokines from activated microglia in the central nervous system contributes to uncontrolled inflammation in neurodegenerative disorders. In this study, we investigated the anti-inflammatory activities of the methylene chloride fraction of JP05 (JP05-MC) on the production of inflammatory mediators in lipopolysaccharide (LPS)-stimulated BV2 mouse microglial cells, and its mechanism of action. JP05-MC significantly inhibited LPS-induced production of NO and the proinflammatory cytokines, TNF-α and IL-6, in BV2 cells. JP05-MC also attenuated the mRNA expression and protein levels of inducible nitric oxide synthase in LPS-induced BV2 cells. JP05-MC significantly attenuated LPS-elicited phosphorylation of the mitogen-activated protein kinase (MAPK), extracellular-signal-regulated kinase 1/2, and nuclear factor-κB (NF-κB) nuclear translocation in BV2 cells. Our results indicate that JP05-MC exerts anti-inflammatory properties via downregulation of inflammatory mediator gene transcription by suppressing the MAPK and NF-κB pathways, suggesting that JP05-MC may have therapeutic potential as an anti-inflammatory agent in neurodegenerative diseases.
- Subjects
DICHLOROMETHANE; INFLAMMATORY mediators; ENZYME activation; MICROGLIA; CYTOKINES; NEURODEGENERATION; BIOCHEMICAL mechanism of action
- Publication
Inflammation, 2012, Vol 35, Issue 1, p332
- ISSN
0360-3997
- Publication type
Article
- DOI
10.1007/s10753-011-9323-7