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- Title
Performance of five serological tests in the diagnosis of visceral and cryptic leishmaniasis: a comparative study.
- Authors
Maritati, Martina; Trentini, Alessandro; Michel, Gregory; Hanau, Stefania; Guarino, Matteo; De Giorgio, Roberto; Pomares, Christelle; Marty, Pierre; Contini, Carlo
- Abstract
Introduction: Leishmaniasis is a major health problem and its diagnosis still represents a challenge. Since consistent evidence on the comparison of serological methods is lacking, our work aims to compare five serological tests for the diagnosis of visceral and asymptomatic leishmaniasis in southern France, a region where leishmaniasis is endemic. Methodology: Serum samples from 75 patients living in Nice, France were retrospectively analyzed. They included patients affected by visceral leishmaniasis (VL; n = 25), asymptomatic carriers (AC; n = 25) and negative controls (n = 25). Each sample was tested using two immunochromatographic tests (ICT; IT LEISH® and TruQuick IgG/IgM®), an indirect fluorescent antibody test (IFAT) and two Western Blotting (WB; LDBio BIORAD® and an in-house method). Results: Diagnosis of VL with IFAT and TruQuick® showed the highest diagnostic performance parameters. IFAT had 100% sensitivity and specificity, while TruQuick had 96% sensitivity and 100% specificity. Finally, the two tests showed high accuracy (100% for IFAT and 98% for TruQuick) for the AC group. WB LDBio® was the only method able to detect Leishmania latent infection, with a sensitivity of 92%, and a specificity of 100%, with a Negative Predictive Value (NPV) of 93%. This performance is reflected in the high accuracy of the test. Conclusions: The data obtained with TruQuick® supports its application in the rapid diagnosis of leishmaniasis in endemic areas, a feature not shown by IFAT despite its high diagnostic performance. Regarding the diagnosis of asymptomatic leishmaniasis, the best results were obtained with WB LDBio®, confirming previous studies.
- Subjects
NICE (France); FRANCE; VISCERAL leishmaniasis; SERODIAGNOSIS; LATENT infection; ANTIBODY titer; LEISHMANIASIS
- Publication
Journal of Infection in Developing Countries, 2023, Vol 17, Issue 5, p693
- ISSN
2036-6590
- Publication type
Article
- DOI
10.3855/jidc.12622