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- Title
Multi-residue analysis using liquid chromatography tandem mass spectrometry for detection of 20 coccidiostats in poultry, livestock, and aquatic tissues.
- Authors
Shun-Hsien Chang; Yu-Hsun Lai; Chih-Neng Huang; Guan-Jhih Peng; Chia-Ding Liao; Ya-Min Kao; Su-Hsiang Tseng; Der-Yuan Wang
- Abstract
In this study, we developed a novel analysis method based on liquid chromatography/ tandem mass spectrometry (LCeMS/MS) to allow the simultaneous identification of 20 coccidiostats in eight matrix categories, including the muscles of chicken, swine, cow, and fish as well as chicken eggs, bovine milk, and porcine viscera. In the pretreatment procedure, acetonitrile/methanol (95:5, v/v) containing 1% formic acid, 5 g of sodium acetate, and 6.0 g of anhydrous magnesium sulfate was used for extraction, followed by a clean-up procedure using n-hexane saturated with ACN to facilitate the elimination of analytes from high lipid samples. Chromatographic separations were achieved using a Poroshell 120SB C18 column and operated with a gradient mobile phase system consisting of methanol (with 0.1% formic acid) and 5 mM ammonium formate, and the MS detection was monitored simultaneously. The method was validated in accordance with the Guidelines for the Validation of Food Chemical Methods by the Taiwan Food and Drug Administration. The limit of quantitation among 8 matrices were 0.5-2 ng g-1. The proposed method proved highly effective in detecting the presence of targeted veterinary drugs, providing a high degree of precision and accuracy over a broad range of matrices.
- Subjects
LIQUID chromatography equipment; EGGS; ACETIC acid; ANALYTICAL biochemistry; ANIMAL experimentation; COLLECTION &; preservation of biological specimens; CATTLE; ANALYTICAL chemistry; FISHES; FOOD chemistry; LIQUID chromatography; MAGNESIUM sulfate; MASS spectrometry; METHANOL; POULTRY; SWINE; ANTIPROTOZOAL agents; ACYCLIC acids
- Publication
Journal of Food & Drug Analysis, 2019, Vol 27, Issue 3, p703
- ISSN
1021-9498
- Publication type
Article
- DOI
10.1016/j.jfda.2019.02.004