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- Title
Regulation of polyphosphate glucokinase gene expression through cotranscriptional processing in Mycobacterium tuberculosis H37Rv.
- Authors
Bokolia, Naveen Prakash; Khan, Inshad Ali
- Abstract
Transcription is a molecular process that involves the synthesis of RNA chain into the 5ʹ–3ʹ direction, and simultaneously nascent RNA chain tends to form geometric structures, known as cotranscriptional folding. This folding determines the functional properties of RNA molecules and possibly has a critical role during the synthesis. This functioning includes the characterized properties of riboswitches and ribozymes, which are significant when the transcription rate is comparable to the cellular environment. This study reports a novel noncoding region important in the genetic expression of polyphosphate glucokinase (ppgk) in Mycobacterium tuberculosis. This noncoding element of ppgk gene undergoes cleavage activity during the transcriptional process in M.tuberculosis. We revealed that cleavage occurs within the nascent RNA, and the resultant cleaved 3ʹRNA fragment carries the Shine–Dalgarno (SD) sequence and expression platform. We concluded cotranscriptional processing at the noncoding region as the required mechanism for ppgk expression that remains constitutive within the bacterial environment. This study defines the molecular mechanism dependent on the transient but highly active structural features of the nascent RNA.
- Subjects
MYCOBACTERIUM tuberculosis; GENE expression; GLUCOKINASE; NON-coding RNA; RIBOSWITCHES; TUBERCULOSIS
- Publication
Journal of Biochemistry, 2021, Vol 170, Issue 5, p593
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/jb/mvab080