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- Title
The mouse FKBP23 binds to BiP in ER and the binding of C-terminal domain is interrelated with Ca<sup>2+</sup> concentration
- Authors
Zhang, Xiaobin; Wang, Ying; Li, Hui; Zhang, Wanqi; Wu, Di; Mi, Huaifeng
- Abstract
FK506 binding protein 23 from mouse (mFKBP23) is a peptidyl-prolyl cis-trans isomerase (PPIase) from the endoplasmic reticulum (ER), which consists of an N-terminal PPIase domain and a C-terminal domain with Ca2+ binding sites. The assay of adsorption from ER extract with glutathione S-transferase-mFKBP23 attached to glutathione-Sepharose 4B shows that mFKBP23 binds to mouse immunoglobulin binding protein (mBiP). The same assay with the recombinant proteins of the N- and C-termini of mFKBP23 shows that the binding of the C-terminus is Ca2+-dependent and the switch point is between 2 and 3 mM. By high concentration of Ca2+ this binding cannot be detected. Furthermore, the Ca2+-regulated binding of mFKBP23 and mBiP in ER can be detected by means of co-immunoprecipitation.
- Subjects
ENDOPLASMIC reticulum; ISOMERASES; CALCIUM ions; PROTEIN binding
- Publication
FEBS Letters, 2004, Vol 559, Issue 1-3, p57
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(04)00024-9