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- Title
Interaction of FLU, a negative regulator of tetrapyrrole biosynthesis, with the glutamyl-tRNA reductase requires the tetratricopeptide repeat domain of FLU
- Authors
Meskauskiene, Rasa; Apel, Klaus
- Abstract
Regulation of tetrapyrrole biosynthesis in plants has been attributed to feedback control of glutamyl-tRNA reductase (GLU-TR) by heme. Recently, another negative regulator, the FLU protein, has been discovered that operates independently of heme. A truncated form of FLU that contains two domains implicated in protein–protein interaction was co-expressed in yeast with either GLU-TR or glutamate-1-semialdehyde-2-1-aminotransferase (GSA-AT), the second enzyme involved in δ-aminolevulinic acid (ALA) biosynthesis. FLU interacts strongly with GLU-TR but not with GSA-AT. Two variants of FLU that carry single amino acid exchanges within their coiled coil and tetratricopeptide repeat (TPR) domains, respectively, were also tested. Only the FLU variant with the mutated TPR motif lost the capacity to interact with GLU-TR.
- Subjects
TETRAPYRROLES; GLUTAMYL-tRNA synthetase
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p27
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03617-7