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- Title
Cytoskeleton-related trafficking of the EAAC1 glutamate transporter after activation of the G<sub>q/11</sub>-coupled neurotensin receptor NTS1
- Authors
Najimi, Mustapha; Maloteaux, Jean-Marie; Hermans, Emmanuel
- Abstract
The possible modulation of the glutamate transporter EAAC1 by a class A G protein-coupled receptor was studied in transfected C6 glioma cells stably expressing the high-affinity neurotensin receptor NTS1. Brief exposure (5 min) to neurotensin increased Na+-dependent D-[3H]aspartate uptake by about 70%. The effect of neurotensin was found to result from an increase in cell surface expression of EAAC1 and accordingly, cytochalasin D and colchicine were shown to block the effect of neurotensin on aspartate uptake, suggesting that the cytoskeleton participates in this regulation. Neither protein kinase C nor phosphatidylinositol 3-kinase activities, two intracellular signaling pathways known to modulate EAAC1, was required for EAAC1-mediated aspartate transport regulation by neurotensin. Together, these results provide evidence for an acute regulation of EAAC1 trafficking after activation of a G protein-coupled receptor.
- Subjects
CYTOSKELETON; OXIDES; NEUROTENSIN; GLIOMAS
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p224
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02981-2