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- Title
Mutation of the RGD sequence does not affect plasma membrane association and growth inhibitory effects of elevated IGFBP-2 in vivo
- Authors
Hoeflich, A.; Reisinger, R.; Vargas, G.A.; Elmlinger, M.W.; Schuett, B.; Jehle, P.M.; Renner-Müller, I.; Lahm, H.; Russo, V.C.; Wolf, E.
- Abstract
Using insulin-like growth factor-binding protein-2 (IGFBP-2) transgenic mice (D mice) as a model of elevated IGFBP-2 expression, which is often found in unphysiological conditions, we found association of IGFBP-2 to purified plasma membranes of many organs. To determine whether the RGD (Arg-Gly-Asp) motif of IGFBP-2 mediates cell surface binding in vivo, we mutated the RGD motif of IGFBP-2 into an RGE (Arg-Gly-Glu) sequence and produced transgenic mice (E mice) which express elevated amounts of mutated IGFBP-2. Our data demonstrate that in vivo IGFBP-2 cell surface association is not dependent on the RGD motif and that mutation of this sequence does not alter growth inhibitory effects of IGFBP-2.
- Subjects
INSULIN-like growth factor-binding proteins; GENETIC mutation; CELL membranes; MICE
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p63
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02935-6