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- Title
Dean-Flow Affected Lateral Focusing and Separation of Particles and Cells in Periodically Inhomogeneous Microfluidic Channels.
- Authors
Bányai, Anita; Farkas, Enikő; Jankovics, Hajnalka; Székács, Inna; Tóth, Eszter Leelőssyné; Vonderviszt, Ferenc; Horváth, Róbert; Varga, Máté; Fürjes, Péter
- Abstract
The purpose of the recent work is to give a better explanation of how Dean vortices affect lateral focusing, and to understand how cell morphology can alter the focusing position compared to spherical particles. The position and extent of the focused region were investigated using polystyrene fluorescent beads with different bead diameters (Ø = 0.5, 1.1, 1.97, 2.9, 4.8, 5.4, 6.08, 10.2, 15.8, 16.5 µm) at different flow rates (0.5, 1, 2 µL/s). Size-dependent focusing generated a precise map of the equilibrium positions of the spherical beads at the end of the periodically altering channels, which gave a good benchmark for focusing multi-dimensional particles and cells. The biological samples used for experiments were rod-shaped Escherichia coli (E. coli), discoid biconcave-shaped red blood cells (RBC), round or ovoid-shaped yeast, Saccharomyces cerevisiae, and soft-irregular-shaped HeLa cancer-cell-line cells to understand how the shape of the cells affects the focusing position at the end of the channel.
- Subjects
ESCHERICHIA coli; CELL morphology; CELL separation; SACCHAROMYCES cerevisiae; COMPUTATIONAL fluid dynamics
- Publication
Sensors (14248220), 2023, Vol 23, Issue 2, p800
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s23020800