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- Title
Locally Synthetized 17-β-Estradiol Reverses Amyloid-β-42-Induced Hippocampal Long-Term Potentiation Deficits.
- Authors
Bellingacci, Laura; Canonichesi, Jacopo; Sciaccaluga, Miriam; Megaro, Alfredo; Mazzocchetti, Petra; Di Mauro, Michela; Costa, Cinzia; Di Filippo, Massimiliano; Pettorossi, Vito Enrico; Tozzi, Alessandro
- Abstract
Amyloid beta 1-42 (Aβ42) aggregates acutely impair hippocampal long-term potentiation (LTP) of synaptic transmission, and 17β-estradiol is crucial for hippocampal LTP. We tested whether boosting the synthesis of neural-derived 17β-estradiol (nE2) saves hippocampal LTP by the neurotoxic action of Aβ42. Electrophysiological recordings were performed to measure dentate gyrus (DG) LTP in rat hippocampal slices. Using a pharmacological approach, we tested the ability of nE2 to counteract the LTP impairment caused by acute exposure to soluble Aβ42 aggregates. nE2 was found to be required for LTP in DG under physiological conditions. Blockade of steroid 5α-reductase with finasteride, by increasing nE2 synthesis from testosterone (T), completely recovered LTP in slices treated with soluble Aβ42 aggregates. Modulation of the glutamate N-methyl-D aspartate receptor (NMDAR) by memantine effectively rescued the LTP deficit observed in slices exposed to Aβ42, and memantine prevented LTP reduction observed under the blocking of nE2 synthesis. nE2 is able to counteract Aβ42-induced synaptic dysfunction. This effect depends on a rapid, non-genomic mechanism of action of nE2, which may share a common pathway with glutamate NMDAR signaling.
- Subjects
LONG-term potentiation; HIPPOCAMPUS (Brain); ASPARTATE receptors; DENTATE gyrus; NEURAL transmission; AMYLOID beta-protein
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 3, p1377
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25031377