We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Human Amnion-Derived Mesenchymal Stromal Cells: A New Potential Treatment for Carbapenem-Resistant Enterobacterales in Decompensated Cirrhosis.
- Authors
Pampalone, Mariangela; Vitale, Giampiero; Gruttadauria, Salvatore; Amico, Giandomenico; Iannolo, Gioacchin; Douradinha, Bruno; Mularoni, Alessandra; Conaldi, Pier Giulio; Pietrosi, Giada
- Abstract
Background: Spontaneous bacterial peritonitis (SBP) is a severe and often fatal infection in patients with decompensated cirrhosis and ascites. The only cure for SBP is antibiotic therapy, but the emerging problem of bacterial resistance requires novel therapeutic strategies. Human amniotic mesenchymal stromal cells (hA-MSCs) possess immunomodulatory and anti-inflammatory properties that can be harnessed as a therapy in such a context. Methods: An in vitro applications of hA-MSCs in ascitic fluid (AF) of cirrhotic patients, subsequently infected with carbapenem-resistant Enterobacterales, was performed. We evaluated the effects of hA-MSCs on bacterial load, innate immunity factors, and macrophage phenotypic expression. Results: hA-MSCs added to AF significantly reduce the proliferation of both bacterial strains at 24 h and diversely affect M1 and M2 polarization, C3a complement protein, and ficolin 3 concentrations during the course of infection, in a bacterial strain-dependent fashion. Conclusion: This study shows the potential usefulness of hA-MSC in treating ascites infected with carbapenem-resistant bacteria and lays the foundation to further investigate antibacterial and anti-inflammatory roles of hA-MSC in in vivo models.
- Subjects
STROMAL cells; CARBAPENEM-resistant bacteria; CIRRHOSIS of the liver; DRUG resistance in bacteria; NATURAL immunity; ASCITIC fluids
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 2, p857
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23020857