We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of Adrenergic Agonists on High-Fat Diet-Induced Hepatic Steatosis in Mice.
- Authors
Nakade, Yukiomi; Kitano, Rena; Yamauchi, Taeko; Kimoto, Satoshi; Sakamoto, Kazumasa; Inoue, Tadahisa; Kobayashi, Yuji; Ohashi, Tomohiko; Sumida, Yoshio; Ito, Kiyoaki; Yoneda, Masashi
- Abstract
The autonomic nervous system, consisting of sympathetic and parasympathetic branches, plays an important role in regulating metabolic homeostasis. The sympathetic nervous system (SNS) regulates hepatic lipid metabolism by regulating adrenergic receptor activation, resulting in the stimulation of hepatic very-low-density lipoprotein-triglyceride (TG) production in vivo. However, only a few studies on the relationship between SNS and hepatic steatosis have been reported. Here, we investigate the effect of adrenergic receptor agonists on hepatic steatosis in mice fed a high-fat diet (HFD). The α-adrenergic receptor agonist phenylephrine (10 mg/kg/d) or the β-adrenergic receptor agonist isoproterenol (30 mg/kg/d) was coadministered with HFD to male mice. After five weeks, hepatic steatosis, TG levels, and hepatic fat metabolism-related biomarkers were examined. HFD treatment induced hepatic steatosis, and cotreatment with phenylephrine, but not isoproterenol, attenuated this effect. Phenylephrine administration upregulated the mRNA levels of hepatic peroxisome proliferator-activated receptor alpha and its target genes (such as carnitine palmitoyltransferase 1) and increased hepatic β-hydroxybutyrate levels. Additionally, phenylephrine treatment increased the expression of the autophagosomal marker LC3-II but decreased that of p62, which is selectively degraded during autophagy. These results indicate that phenylephrine inhibits hepatic steatosis through stimulation of β-oxidation and autophagy in the liver.
- Subjects
ADRENERGIC agonists; FATTY degeneration; SYMPATHETIC nervous system; PEROXISOME proliferator-activated receptors; AUTONOMIC nervous system; ADRENERGIC receptors
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 24, p9392
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21249392