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- Title
Photoimmunotherapy Using Cationic and Anionic Photosensitizer-Antibody Conjugates against HIV Env-Expressing Cells.
- Authors
Sadraeian, Mohammad; Bahou, Calise; da Cruz, Edgar Ferreira; Janini, Luíz Mário Ramos; Sobhie Diaz, Ricardo; Boyle, Ross W.; Chudasama, Vijay; Eduardo Gontijo Guimarães, Francisco
- Abstract
Different therapeutic strategies have been investigated to target and eliminate HIV-1-infected cells by using armed antibodies specific to viral proteins, with varying degrees of success. Herein, we propose a new strategy by combining photodynamic therapy (PDT) with HIV Env-targeted immunotherapy, and refer to it as HIV photoimmunotherapy (PIT). A human anti-gp41 antibody (7B2) was conjugated to two photosensitizers (PSs) with different charges through different linking strategies; "Click" conjugation by using an azide-bearing porphyrin attached via a disulfide bridge linker with a drug-to-antibody ratio (DAR) of exactly 4, and "Lysine" conjugation by using phthalocyanine IRDye 700DX dye with average DARs of 2.1, 3.0 and 4.4. These photo-immunoconjugates (PICs) were compared via biochemical and immunological characterizations regarding the dosimetry, solubility, and cell targeting. Photo-induced cytotoxicity of the PICs were compared using assays for apoptosis, reactive oxygen species (ROS), photo-cytotoxicity, and confocal microscopy. Targeted phototoxicity seems to be primarily dependent on the binding of PS-antibody to the HIV antigen on the cell membrane, whilst being independent of the PS type. This is the first report of the application of PIT for HIV immunotherapy by killing HIV Env-expressing cells.
- Subjects
REACTIVE oxygen species; VIRAL antibodies; HIV; VIRAL proteins; PHOTODYNAMIC therapy
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 23, p9151
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21239151